Instruction : Trio-Smart® Breath Test

Trio-Smart Breath Test Instructions

Comprehensive breath testing instructions designed to help ensure accurate sample collection and reliable results for your Trio-Smart® Breath Test.

What's Included in Your Test Kit?

Before getting started, confirm that your kit contains all required components:

One plastic bag for the secure return of breath samples
Nine breath collection bags with white valves
Nine sample labels to complete and attach to each bag
One mouthpiece for breath collection
One requisition form
One prepaid return shipping label

Prepare Your Sugar Substrate Drink

Your healthcare provider has selected the substrate drink required for your test.

If You Were Prescribed Lactulose

You may receive a signed prescription form in your Trio-Smart kit, or your healthcare provider may send it directly to your pharmacy. Fill the prescription before your testing day.

After collecting your first breath sample, consume a single dose of 10gm lactulose in a 15ml solution (approximately one tablespoon) with or followed by one cup (8oz) of water. Discard any remaining solution.

If You Received Glucose

If your kit includes a glucose solution, consume the entire 75 g dose after collecting your first breath sample.

Important Pre-Test Preparation

Proper preparation is essential to help ensure accurate and reliable results.

Medication Considerations

If you are currently taking antibiotics, consult your healthcare provider before testing.

If tolerated, promotility medications and laxatives should be discontinued at least 7 days before sample collection.

Follow the Restricted Diet

On the day before testing, consume only the following foods: Meat (beef, pork, poultry, fish), Tofu, Eggs, Potatoes, and White rice. Seasonings should be limited to oil, Salt, and Pepper. Permitted beverages include water, plain coffee and plain tea.

Do not consume fruits, vegetables, or any foods and beverages not listed above.

Complete the Fasting Period

Observe an 8–12 hour fasting period before collecting your first sample. During sample collection, do not eat or drink anything except the prescribed lactulose solution with one cup of water, if applicable. Additional small sips of water may be taken if needed.

Complete Your Requisition Form

Option 1: Prepaid Testing

If your Trio-Smart® test has already been prepaid, proceed directly to sample collection preparation.

Option 2: Insurance Processing

Verify that all insurance and policyholder information is complete and accurate.
Include copies of both sides of your insurance card.
Alternatively, insurance card images may be emailed to support@triosmartbreath.com.
Sign and date the requisition form where indicated.

Remember to include the completed requisition form and insurance information when returning your samples.

Sample Collection Instructions

Follow these steps carefully to ensure proper collection of the breath sample.

Step 1: Label Your Collection Bag

Complete a sample label with:

Sample number
Collection time
Collection date
Full name
Date of birth

Attach the label to a deflated collection bag.

Step 2: Attach the Mouthpiece

Securely attach the mouthpiece to the valve. Ensure the small hole on the mouthpiece is facing upward.

Turn the valve cap counterclockwise until it reaches the fully open position.

Step 3: Collect Your Breath Sample

Take a deep breath and exhale continuously into the mouthpiece.

Air will initially escape through the small opening at the top. After approximately two seconds, cover the opening with your finger while continuing to exhale, allowing the bag to inflate.

Only one breath should be collected per bag.

Step 4: Seal the Collection Bag

Turn the valve cap clockwise until fully closed.

Remove the mouthpiece from the valve after sealing the bag.

Step 5: Consume the Substrate Drink

After collecting your first sample, consume your prescribed substrate drink.

Lactulose: 10gm in a 15ml solution with or followed by one cup (8oz) of water.
Glucose: Consume the full 75 g dose provided. Wait 15 minutes before collecting the next sample.

Step 6: Collect Remaining Samples

Repeat Steps 1–4 for the remaining eight breath collection bags.

Allow 15 minutes between each sample collection and ensure every label is completed accurately with the correct collection time.

Return Your Samples

Once all samples have been collected:

Place all filled collection bags inside the provided plastic bag and seal it securely.
Place the sealed plastic bag inside the Trio-Smart® box.
Include your signed requisition form and, if required, your insurance information.
Cover the original shipping label with the prepaid return label provided.
Seal the box securely with packing tape.
Return your kit to the shipping provider listed on the prepaid label within 24 hours of completing your test, or the next business day if testing is completed over a weekend.

Results are typically made available to your healthcare provider within 7 business days after the laboratory receives your samples.

For your records, keep your shipping tracking number until delivery is confirmed.

Receiving Your Results

Ready to Complete Your Trio-Smart® Test?

Follow the instructions carefully for accurate sample collection and reliable diagnostic results.

Access the complete Trio-Smart Breath Test Instructions PDF for more details, before you begin testing – TRI Smart sample collection instructions!

FAQs

How long does the Trio-Smart® Breath Test take?

The test involves collecting nine breath samples over a scheduled period with 15-minute intervals between each collection.

Can I drink water during the test?

Only limited water is permitted during testing. Follow the provided instructions carefully to avoid affecting your results.

What foods can I eat the day before testing?

Only approved foods such as meat, tofu, eggs, potatoes, white rice, and limited seasonings should be consumed during the preparation diet.

What happens if I forget to complete a sample label?

Each sample must be accurately labeled with the required information. Missing or incorrect labels may delay processing or affect sample identification.

When will my healthcare provider receive my results?

Results are generally available to your healthcare provider within 7 business days after the laboratory receives your completed test kit.